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Knockdown of corticotropin-releasing factor 1 receptors in the ventral tegmental area enhances conditioned fear
journal contribution
posted on 2016-09-01, 00:00 authored by N A Chen, D E Ganella, R A D Bathgate, A Chen, A J Lawrence, Jee Hyun KimJee Hyun KimThe neuropeptide corticotropin-releasing factor (CRF) coordinates the physiological and behavioural responses to stress. CRF receptors are highly expressed in the ventral tegmental area (VTA), an important region for motivated behaviour. Therefore, we examined the role of CRF receptor type 1 (CRFR1) in the VTA in conditioned fear, using a viral-mediated RNA interference approach. Following stereotaxic injection of a lentivirus that contained either shCRF-R1 or a control sequence, mice received tone-footshock pairings. Intra-VTA shCRF-R1 did not affect tone-elicited freezing during conditioning. Once conditioned fear was acquired, however, shCRF-R1 mice consistently showed stronger freezing to the tone even after extinction and reinstatement. These results implicate a novel role of VTA CRF-R1 in conditioned fear, and suggest how stress may modulate aversive learning and memory.
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Journal
European neuropsychopharmacologyVolume
26Issue
9Pagination
1533 - 1540Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0924-977XeISSN
1873-7862Language
engPublication classification
C1 Refereed article in a scholarly journalUsage metrics
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