Deakin University
Browse
duan-lef1enhancesthe-2021.pdf (1.28 MB)

Lef1 enhances the progression of colonic adenocarcinoma via remodeling the cell motility associated structures

Download (1.28 MB)
journal contribution
posted on 2021-10-01, 00:00 authored by L Xiao, C Zhang, X Li, C Jia, L Chen, Y Yuan, Q Gao, Z Lu, Y Feng, R Zhao, X Zhao, S Cheng, Z Shu, J Xu, Wei DuanWei Duan, G Nie, Y Hou
Lymphoid enhancer-binding factor 1 (LEF1) is a key transcription factor mediating the Wnt signaling pathway. LEF1 is a regulator that is closely associated with tumor malignancy and is usually upregulated in cancers, including colonic adenocarcinoma. The underlying molecular mechanisms of LEF1 regulation for colonic adenocarcinoma progression remain unknown. To explore it, the LEF1 expression in caco2 cells was inhibited using an shRNA approach. The results showed that downregulation of LEF1 inhibited the malignancy and motility associated microstructures, such as polymerization of F-actin, β-tubulin, and Lamin B1 in caco2 cells. LEF1 inhibition suppressed the expression of epithelial/endothelial-mesenchymal transition (EMT) relevant genes. Overall, the current results demonstrated that LEF1 plays a pivotal role in maintaining the malignancy of colonic adenocarcinoma by remodeling motility correlated microstructures and suppressing the expression of EMT-relevant genes. Our study provided evidence of the roles LEF1 played in colonic adenocarcinoma progression, and suggest LEF1 as a potential target for colonic adenocarcinoma therapy

History

Journal

International Journal of Molecular Sciences

Volume

22

Issue

19

Article number

10870

Pagination

1 - 14

Publisher

MDPI

Location

Basel, Switzerland

ISSN

1661-6596

eISSN

1422-0067

Language

eng

Publication classification

C1 Refereed article in a scholarly journal