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MERP1: a mammalian ependymin-related protein gene differentially expressed in hematopoietic cells
journal contribution
posted on 2002-03-20, 00:00 authored by C C Gregorio-King, J L McLeod, Fiona Collier, G R Collier, Kristy BoltonKristy Bolton, G J Van Der Meer, J Apostolopoulos, M A KirklandWe have utilized differential display polymerase chain reaction to investigate the gene expression of hematopoietic progenitor cells from adult bone marrow and umbilical cord blood. A differentially expressed gene was identified in CD34+ hematopoietic progenitor cells, with low expression in CD34- cells. We have obtained the full coding sequence of this gene which we designated human mammalian ependymin-related protein 1 (MERP1). Expression of MERP1 was found in a variety of normal human tissues, and is 4- and 10-fold higher in adult bone marrow and umbilical cord blood CD34+ cells, respectively, compared to CD34- cells. Additionally, MERP1 expression in a hematopoietic stem cell enriched population was down-regulated with proliferation and differentiation. Conceptual translation of the MERP1 open reading frame reveals significant homology to two families of glycoprotein calcium-dependant cell adhesion molecules: ependymins and protocadherins.
History
Journal
Gene : an international journal on genes and genomesVolume
286Issue
2Pagination
249 - 257Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0378-1119Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2002, Elsevier Science B.V.Usage metrics
Categories
Keywords
AdultAmino Acid SequenceAntigens, CD34Base SequenceBlotting, NorthernBone Marrow CellsChromosome MappingChromosomes, Human, Pair 7Computational BiologyDNA, ComplementaryFetal BloodGene Expression RegulationHematopoietic Stem CellsHumansMolecular Sequence DataNeoplasm ProteinsNerve Tissue ProteinsSequence AlignmentSequence Analysis, DNASequence Homology, Amino AcidScience & TechnologyLife Sciences & BiomedicineGenetics & Hereditybone marrowumbilical cord bloodCD34(+)protocadherinSTEM-CELLSEXTRACELLULAR-MATRIXBRAINADHESIONBINDINGBIOLOGYREGIONSFAMILYPhysiologyGenetics
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