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Mesoporous silica nanorods toward efficient loading and intracellular delivery of siRNA
journal contribution
posted on 2018-02-01, 00:00 authored by L Chen, X She, T Wang, Sarah ShigdarSarah Shigdar, Wei DuanWei Duan, Lingxue KongLingxue KongThe technology of RNA interference (RNAi) that uses small interfering RNA (siRNA) to silence the gene expression with complementary messenger RNA (mRNA) sequence has great potential for the treatment of cancer in which certain genes were usually found overexpressed. However, the carry and delivery of siRNA to the target site in the human body can be challenging for this technology to be used clinically to silence the cancer-related gene expression. In this work, rod shaped mesoporous silica nanoparticles (MSNs) were developed as siRNA delivery system for specific intracellular delivery. The rod MSNs with an aspect ratio of 1.5 had a high surface area of 934.28 m2/g and achieved a siRNA loading of more than 80 mg/g. With the epidermal growth factor (EGF) grafted on the surface of the MSNs, siRNA can be delivered to the epidermal growth factor receptor (EGFR) overexpressed colorectal cancer cells with high intracellular concentration compared to MSNs without EGF and lead to survivin gene knocking down to less than 30%.
History
Journal
Journal of nanoparticle researchVolume
20Article number
37Pagination
1 - 13Publisher
SpringerLocation
Cham, SwitzerlandPublisher DOI
ISSN
1388-0764eISSN
1572-896XLanguage
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2017, Springer Science+Business Media B.V., part of Springer NatureUsage metrics
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No categories selectedKeywords
MSNssiRNAEGFIntracellular deliveryGene silencingNanomedicineScience & TechnologyPhysical SciencesTechnologyChemistry, MultidisciplinaryNanoscience & NanotechnologyMaterials Science, MultidisciplinaryChemistryScience & Technology - Other TopicsMaterials ScienceSMALL INTERFERING RNADRUG-RESISTANCEMULTIDRUG-RESISTANCEENHANCED GENECO-DELIVERYNANOPARTICLESENDOCYTOSISDOXORUBICINCHEMOTHERAPYMETASTASIS
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