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Oxidised mannan as a novel adjuvant inducing mucosal IgA production.
journal contribution
posted on 2002-01-15, 00:00 authored by John StambasJohn Stambas, G Pietersz, I McKenzie, C CheersMannan, oxidatively coupled to recombinant protein antigens, has here been tested as a possible adjuvant for the production of antibody on the mucosa. Given intranasally, but not intraperitoneally, mannan markedly enhanced the production of IgA, IgG1 and IgG2a in the serum, and IgA locally in the lung and at remote mucosal sites, including tears, vaginal and salivary secretions. Oxidative coupling was critical to its action, since neither mannan simply mixed with protein nor mannan–protein conjugates which had been reduced by treatment with sodium borohydride, acted as adjuvants. Oxidatively coupled mannan was compared with the widely studied mucosal adjuvant, cholera toxin (CT). The use of oxidised mannan as an adjuvant induced better responses than CT judged by the induction of IgA in serum, vaginal washings and saliva. Thus, oxidised mannan, which is non-toxic and can be administered without injection, is a suitable adjuvant coupled with protective antigens for vaccinating against a number of infections that occur via the mucous membranes.
History
Journal
VaccineVolume
20Issue
7-8Pagination
1068 - 1078Publisher
Elsevier Ltd.Location
Guildford, EnglandPublisher DOI
ISSN
0264-410XeISSN
1873-2518Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
Copyright 2002 Elsevier Science Ltd. All rights reserved.Usage metrics
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mucosaIgAoxidised mannanScience & TechnologyLife Sciences & BiomedicineImmunologyMedicine, Research & ExperimentalResearch & Experimental MedicineSECRETORY IMMUNOGLOBULIN-ASURFACE PROTEIN ANTIGENTOXIN-B SUBUNITIMMUNE-RESPONSESCHOLERA-TOXINESCHERICHIA-COLIMANNOSE RECEPTORDENDRITIC CELLSINTRANASAL IMMUNIZATIONINFLUENZA-VIRUS
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