duan-peptideflurorescent-2013.pdf (2.2 MB)
Peptide-fluorescent bacteria complex as luminescent reagents for cancer diagnosis
journal contribution
posted on 2013-01-01, 00:00 authored by B Dong, A Wang, L Yuan, L Chen, K Pu, Wei DuanWei Duan, X Yan, Y ZhuCurrently in clinic, people use hematoxylin and eosin stain (H&E stain) and immunohistochemistry methods to identify the generation and genre of cancers for human pathological samples. Since these methods are inaccurate and time consuming, developing a rapid and accurate method to detect cancer is urgently demanded. In our study, binding peptides for lung cancer cell line A549 were identified using bacteria surface display method. With those binding peptides for A549 cells on the surface, the fluorescent bacteria (Escherichia coli with stably expressed green fluorescent protein) were served as specific detecting reagents for the diagnosis of cancers. The binding activity of peptide-fluorescent bacteria complex was confirmed by detached cancer cells, attached cancer cells and mice tumor xenograft samples. A unique fixation method was developed for peptide-bacteria complex in order to make this complex more feasible for the clinic use. This peptide-fluorescent bacteria complex has great potential to become a new diagnostic tool for clinical application.
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Journal
PLOS OneVolume
8Issue
1Season
Article e54467Publisher
Public Library of ScienceLocation
San Francisco, CaliforniaPublisher DOI
ISSN
1932-6203Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2013, Public Library of ScienceUsage metrics
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