File(s) under permanent embargo
Secretion of β-lactamase from K. lactis using pEPS1, a convenient episomal vector designed for the secretion of foreign proteins
journal contribution
posted on 1993-03-01, 00:00 authored by I G Macreadie, L A Castelli, Alister WardAlister Ward, M J R Stark, A A AzadA convenient shuttle vector that enables high level secretion of proteins from Kluyveromyces lactis has been developed. The vector, pEPS1, contains a unique cloning site that allows the construction, in a single ligation step, of episomal plasmids capable of directing secretion of foreign gene products from K. lactis. As an example we demonstrate the production of β-lactamase and determine optimal conditions for its secretion into the culture media.
History
Journal
Biotechnology lettersVolume
15Issue
3Pagination
213 - 218Publisher
Kluwer Academic PublishersLocation
Dordrecht, The NetherlandsPublisher DOI
ISSN
0141-5492eISSN
1573-6776Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
1993, Science and Technology LettersUsage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC