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Secretion of the glucose-regulated selenoprotein SEPS1 from hepatoma cells
journal contribution
posted on 2007-05-11, 00:00 authored by Yuan Gao, J Pagnon, Helen Feng, Nicky Konstantopoulos, Jeremy Jowett, Ken WalderKen WalderSEPS1 (also called selenoprotein S, SelS, Tanis or VIMP) is a selenoprotein, localized predominantly in the ER membrane and also on the cell surface. In this report, we demonstrate that SEPS1 protein is also secreted from hepatoma cells but not from five other types of cells examined. The secretion can be abolished by the ER-Golgi transport inhibitor Brefeldin A and by the protein synthesis inhibitor cycloheximide. Using a sandwich ELISA, SEPS1 was detected in the sera of 65 out of 209 human subjects (31.1%, average = 15.7 ± 1.1 ng/mL). Fractionation of human serum indicated that SEPS1 was associated with LDL and possibly with VLDL. The function of plasma SEPS1 is unclear but may be related to lipoprotein metabolism.
History
Journal
Biochemical and biophysical research communicationsVolume
356Issue
3Pagination
636 - 641Publisher
Academic PressLocation
Orlando, Fla.Publisher DOI
ISSN
0006-291XeISSN
1090-2104Language
engPublication classification
C1 Refereed article in a scholarly journal; C Journal articleCopyright notice
2007, ElsevierUsage metrics
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