File(s) under permanent embargo
Sensitivity of live microalgal aquaculture feed to singlet oxygen-based photodynamic therapy
journal contribution
posted on 2019-12-01, 00:00 authored by D Malara, L Høj, M Oelgemöller, Martino MalerbaMartino Malerba, G Citarrella, K HeimannHighly nutritional microalgal species are extensively used in aquaculture as live feedstock. Due to difficulties in maintaining microalgae in axenic conditions, they represent a potential pathogen carrier and disease vector in aquaculture ponds. Photodynamic therapy (PDT) via singlet oxygen (1O2) production is a promising sterilization technique in aquaculture. Here, we report on the sensitivity of aquaculture-relevant microalgae towards 1O2 generated by the cationic photosensitizer TMPyP. Possible PDT sterilization protocols of contaminated microalgae cultures were evaluated using the luminescent bacterium Vibrio campbellii ISO7 as a model aquaculture pathogen. Species-specific sensitivity of microalgae to TMPyP-mediated PDT was demonstrated and found to be strongly influenced by the nature and architecture of their respective cell wall. While cytotoxicity was not evident against Nannochloropsis oculata, toxicity of 1O2 was dose-, time- and light activation-dependent against Tisochrysis lutea, Tetraselmis chui, Chaetoceros muelleri and Picochlorum atomus. The 1O2-resilient N. oculata was sterilized when incubated under light in the presence of V. campbellii ISO7 (up to 107 CFU mL−1) and 20 μM TMPyP; hence, TMPyP-based PDT sterilization of N. oculata could be suitable for aquaculture hatcheries. This study also suggests that PDT using cationic porphyrins such as TMPyP holds potential as an algicidal treatment in aquaria and aquaculture systems (but more research using opportunistic and toxic species is needed for confirmation).
History
Journal
Journal of applied phycologyVolume
31Pagination
3593 - 3606Publisher
SpringerLocation
[Dordrecht, The Netherlands]Publisher DOI
ISSN
0921-8971eISSN
1573-5176Language
engPublication classification
C Journal article; C1.1 Refereed article in a scholarly journalUsage metrics
Categories
Keywords
MicroalgaePhotoinactivationSterilizationAquaculture live feedPhotodynamic antimicrobial chemotherapyScience & TechnologyLife Sciences & BiomedicineBiotechnology & Applied MicrobiologyMarine & Freshwater BiologyAXENIC CULTURESMARINE LARVICULTUREHYDROGEN-PEROXIDEWATER-TREATMENTMETHYLENE-BLUEVIBRIO-HARVEYIGROWTH-RATESCELL-WALLALGAEINACTIVATIONPlant Biology
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC