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Sindbis virus vectors elicit hemagglutinin-specific humoral and cellular immune responses and offer a dose-sparing strategy for vaccination
journal contribution
posted on 2008-10-16, 00:00 authored by A Miller, R Center, John StambasJohn Stambas, G Deliyannis, P Doherty, J Howard, S Turner, D PurcellWe report here on the use of a Sindbis virus-based DNA-launch RNA replicon vector (pSIN-HA) that expresses influenza hemagglutinin (HA) as an immunogen. Immunization of mice with pSIN-HA generated anti-HA antibody and CTL responses and resulted in lower lung viral titers after influenza challenge when compared to controls. Importantly, immunization with a low dose of pSIN-HA mediated significantly reduced lung viral titers following challenge at 43 weeks after the final immunization. In contrast, immunization with a non-replicon DNA vector expressing HA failed to mediate reduced lung viral titer at the same dose. This demonstrated the dose-sparing capacity of the SIN vector system and its ability to stimulate long-term memory responses, properties that are highly desirable in any vaccine formulation.
History
Journal
VaccineVolume
26Issue
44Pagination
5641 - 5648Publisher
Elsevier Ltd.Location
Guildford, EnglandPublisher DOI
ISSN
0264-410XeISSN
1873-2518Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
Copyright 2008 Elsevier Ltd All rights reserved.Usage metrics
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