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Solubility and Stability of cytochrome c in hydrated ionic liquids : effect of oxo acid residues and kosmotropicity
journal contribution
posted on 2007-07-01, 00:00 authored by K Fujita, D MacFarlane, Maria ForsythMaria Forsyth, M Yoshizawa-Fujita, K Murata, N Nakamura, H OhnoHydrated ionic liquids (ILs) were prepared by adding appropriate amounts of water to hydrophilic ILs. Some hydrated ILs show excellent solubilizing ability for proteins, keeping the basic properties of ILs. The solubility of cytochrome c (cyt c) depended on the structure of the component ions. When component anions have oxo acid residues, the resulting hydrated ILs solubilize cyt c quite well. In such hydrated ILs, the structure and activity of cyt c is influenced by the kosmotropicity of the component ions. We synthesized ILs from various ions having different kosmotropicity, including dihydrogen phosphate (dhp), dibutylphosphate, acetate, lactate, and methanesulfonate as anions. The activity of the dissolved cyt c depends on the permutations of kosmotropicity of the component ions. cyt c shows no structural change and retains its activity when dissolved in the hydrated choline dhp, which is an excellent combination of chaotropic cation and kosmotropic anion. Furthermore, cyt c dissolved in the hydrated choline dhp remained in a native state and was active after 18 months of storage at room temperature.
History
Journal
BiomacromoleculesVolume
8Issue
7Pagination
2080 - 2086Publisher
American Chemical SocietyLocation
Washington, D.C.Publisher DOI
ISSN
1525-7797eISSN
1526-4602Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2007, American Chemical SocietyUsage metrics
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