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Subcellular localization of Aleutian mink disease parvovirus proteins and DNA during permissive infection of Crandell feline kidney cells

journal contribution
posted on 1996-05-01, 00:00 authored by M B Oleksiewicz, F Costello, M Huhtanen, J B Wolfinbarger, Soren AlexandersenSoren Alexandersen, M E Bloom
Confocal microscopy allowed us to localize viral nonstructural (NS) and capsid (VP) proteins and DNA simultaneously in cells permissively infected with Aleutian mink disease parvovirus (ADV). Early after infection, NS proteins colocalized with viral DNA to form intranuclear inclusions, whereas VP proteins formed hollow intranuclear shells around the inclusions. Later, nuclei had irregular outlines and were virtually free of ADV products. In these cells, inclusions of viral DNA with or without associated NS protein were embedded in cytoplasmic VP protein. These findings implied that ADV replication within an infected cell is regulated spatially as well as temporally.

History

Journal

Journal of virology

Volume

70

Issue

5

Pagination

3242 - 3247

Publisher

American Society for Microbiology

Location

Washington, D.C.

ISSN

0022-538X

eISSN

1098-5514

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

1996, American Society for Microbiology