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Subcellular localization of Aleutian mink disease parvovirus proteins and DNA during permissive infection of Crandell feline kidney cells
journal contribution
posted on 1996-05-01, 00:00 authored by M B Oleksiewicz, F Costello, M Huhtanen, J B Wolfinbarger, Soren AlexandersenSoren Alexandersen, M E BloomConfocal microscopy allowed us to localize viral nonstructural (NS) and capsid (VP) proteins and DNA simultaneously in cells permissively infected with Aleutian mink disease parvovirus (ADV). Early after infection, NS proteins colocalized with viral DNA to form intranuclear inclusions, whereas VP proteins formed hollow intranuclear shells around the inclusions. Later, nuclei had irregular outlines and were virtually free of ADV products. In these cells, inclusions of viral DNA with or without associated NS protein were embedded in cytoplasmic VP protein. These findings implied that ADV replication within an infected cell is regulated spatially as well as temporally.
History
Journal
Journal of virologyVolume
70Issue
5Pagination
3242 - 3247Publisher
American Society for MicrobiologyLocation
Washington, D.C.ISSN
0022-538XeISSN
1098-5514Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
1996, American Society for MicrobiologyUsage metrics
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No categories selectedKeywords
Aleutian Mink Disease VirusAnimalsCapsidCatsCell LineDNA, ViralKidneyMicroscopy, ConfocalSubcellular FractionsTime FactorsViral Nonstructural ProteinsVirus ReplicationScience & TechnologyLife Sciences & BiomedicineVirologyMINUTE VIRUSNUCLEOTIDE-SEQUENCEVIRAL REPLICATIONBINDING PROTEINMURINE CELLSADULT MINKRAT-CELLSMICEEXPRESSIONIDENTIFICATION
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