Deakin University
Browse

File(s) under permanent embargo

Sulfatide-tenascin interaction mediates binding to the extracellular matrix and endocytic uptake of liposomes in glioma cells

journal contribution
posted on 2007-02-01, 00:00 authored by K Shao, Q Hou, M Go, Wei DuanWei Duan, Steve Cheung, S Feng, K Wong, A Yoram, W Zhang, Z Huang, Q Li
Tenascin-C is an extracellular matrix glycoprotein, whose expression is highly restricted in normal adult tissues, but markedly up-regulated in a range of tumors, and therefore serves as a potential receptor for targeted anticancer drug or gene delivery. We describe here a liposomal carrier system in which the targeting ligand is sulfatide. Experiments with tenascin-C-expressing glioma cells demonstrated that binding of liposomes to the extracellular matrix relied essentially on the sulfatide-tenascin-C interaction. Following binding to the extracellular matrix, the sulfatide-containing liposomes were internalized via both caveolae/lipid raft- and clathrin-dependent pathways, which would ensure direct cytoplasmic release of the cargoes carried in the liposomes. Such natural lipid-guided intracellular delivery targeting at the extracellular matrix glycoproteins of tumor cells thus opens a new direction for development of more effective anticancer chemotherapeutics in future.

History

Journal

Cellular and molecular life sciences

Volume

64

Issue

4

Pagination

506 - 515

Publisher

Birkhaeuser Verlag

Location

Basel, Switzerland

ISSN

1420-682X

eISSN

1420-9071

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2007, Birkhaeuser Verlag, Basel