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Surface-engineered biocatalytic composite membranes for reduced protein fouling and self-cleaning
journal contribution
posted on 2018-01-01, 00:00 authored by Anbu VanangamudiAnbu Vanangamudi, Daisuke Saeki, Ludovic Dumee, Mikel Duke, Todor Vasiljevic, Hideto Matsuyama, Xing YangA new biocatalytic nanofibrous composite ultrafiltration membrane was developed to reduce protein fouling interactions and self-clean the membrane surface. The dual-layer poly(vinylidenefluoride)/nylon-6,6/chitosan composite membrane contains a hydrophobic poly(vinylidenefluoride) cast support layer and a hydrophilic functional nylon-6,6/chitosan nanofibrous surface layer where enzymes were chemically attached. The intrinsic surface chemistry and high surface area of the nanofibers allowed optimal and stable immobilization of trypsin (TR) and α-chymotrypsin enzymes via direct covalent binding. The enzyme immobilization was confirmed by X-ray photoelectron spectroscopy and visualized by confocal microscopy analysis. The prepared biocatalytic composite membranes were nanoporous with superior permeability offering stable protein antiadhesion and self-cleaning properties owing to the repulsive mechanism and digestion of proteins into peptides and amino acids, which was quantified by the gel electrophoresis technique. The TR-immobilized composite membranes exhibited 2.7-fold higher permeance and lower surface protein contamination with 3-fold greater permeance recovery, when compared to the pristine membrane after two ultrafiltration cycles with the model feed solution containing bovine serum albumin/NaCl/CaCl2. The biocatalytic membranes retained about 50% of the enzyme activity after six reuse cycles but were regenerated to 100% activity after enzyme reloading, leading to a simple and cost-effective water remediation operation. Such surface- and pore-engineered membranes with self-cleaning properties offer a viable solution for severe surface protein contamination in food and water applications.
History
Journal
Applied materials & interfacesVolume
10Issue
32Pagination
27477 - 27487Publisher
American Chemical SocietyLocation
Washington, D.C.Publisher DOI
eISSN
1944-8252Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2018, American Chemical SocietyUsage metrics
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No categories selectedKeywords
antifoulingbiocatalystsenzymesnanofibersultrafiltrationScience & TechnologyTechnologyNanoscience & NanotechnologyMaterials Science, MultidisciplinaryScience & Technology - Other TopicsMaterials ScienceULTRAFILTRATION MEMBRANECOVALENT IMMOBILIZATIONPOLY(ETHYLENE GLYCOL)ENZYME IMMOBILIZATIONALPHA-CHYMOTRYPSINTRYPSINPERFORMANCEPOLYANILINEMECHANISMS
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