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The ADAMTS5 metzincin regulates zebrafish somite differentiation

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journal contribution
posted on 2018-03-07, 00:00 authored by Carolyn Dancevic, Yann Gibert, Joachim Berger, Adam D Smith, Clifford LiongueClifford Liongue, Nicole Stupka, Alister WardAlister Ward, Daniel R McCulloch
The ADAMTS5 metzincin, a secreted zinc-dependent metalloproteinase, modulates the extracellular matrix (ECM) during limb morphogenesis and other developmental processes. Here, the role of ADAMTS5 was investigated by knockdown of zebrafish adamts5 during embryogenesis. This revealed impaired Sonic Hedgehog (Shh) signaling during somite patterning and early myogenesis. Notably, synergistic regulation of myod expression by ADAMTS5 and Shh during somite differentiation was observed. These roles were not dependent upon the catalytic activity of ADAMTS5. These data identify a non-enzymatic function for ADAMTS5 in regulating an important cell signaling pathway that impacts on muscle development, with implications for musculoskeletal diseases in which ADAMTS5 and Shh have been associated.

History

Journal

International journal of molecular sciences

Volume

19

Issue

3

Article number

766

Pagination

1 - 12

Publisher

MDPI

Location

Basel, Switzerland

ISSN

1422-0067

eISSN

1422-0067

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2018, the authors