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The control of epidermal growth factor grafted on mesoporous silica nanoparticles for targeted delivery
journal contribution
posted on 2015-08-07, 00:00 authored by Xiaodong She, Lijue Chen, L Velleman, Chengpeng Li, C He, J Denman, Tao Wang, Sarah ShigdarSarah Shigdar, Wei DuanWei Duan, Lingxue KongLingxue KongThe performance of biomaterials in a biological environment is largely influenced by the surface properties of the biomaterials. In particular, grafted targeting ligands significantly impact the subsequent cellular interactions. The utilisation of a grafted epidermal growth factor (EGF) is effective for targeted delivery of drugs to tumours, but the amount of these biological attachments cannot be easily quantified as most characterization methods could not detect the extremely low amount of EGF ligands grafted on the surface of nanoparticles. In this study, hollow mesoporous silica nanoparticles (HMSNs) were functionalized with amine groups to conjugate with EGFs via carbodiimide chemistry. Time of flight secondary ion mass spectrometry (ToF-SIMS), a very surface specific technique (penetration depth <1.5 nm), was employed to study the binding efficiency of the EGF to the nanoparticles. Principal component analysis (PCA) was implemented to track the relative surface concentrations of EGFs on HMSNs. It was found that ToF-SIMS combined with the PCA technique is an effective method to evaluate the immobilization efficiency of EGFs. Based on this useful technique, the quantity and density of the EGF attachments that grafted on nanoparticles can be effectively controlled by varying the EGF concentration at grafting stages. Cell experiments demonstrated that the targeting performance of EGFR positive cells was affected by the number of EGFs attached on HMSNs.
History
Journal
Journal of materials chemistry BVolume
3Issue
29Pagination
6094 - 6104Publisher
Royal Society of ChemistryLocation
Cambridge, Eng.Publisher DOI
ISSN
2050-7518eISSN
2050-750XLanguage
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2015, Royal Society of ChemistryUsage metrics
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