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The development of genetic tools for dissecting the biology of malaria parasites
journal contribution
posted on 2000-01-01, 00:00 authored by Tania De Koning-WardTania De Koning-Ward, C J Janse, A P WatersPlasmodium parasites are haploid unicellular organisms that cause malaria. In the last decade, transfection systems have been developed for both human and animal model species of Plasmodium, providing a broad range of genetic tools for the study of malaria parasite biology. Transient transfection has been used to provide insight into the regulation of gene expression by Plasmodium spp. The development of stable transfection technologies has provided the opportunity to express transgenes in Plasmodium spp., as well as elucidate the function of proteins by disrupting, modifying, or replacing the genes encoding them. These genetic tools represent an important breakthrough for malaria research and will significantly contribute to our understanding of the biology of the parasite. However, further developments in this technology are still required, especially because the full genome sequence of the major human malaria parasite Plasmodium falciparum will shortly be available. Ultimately, the biological information obtained through genetic manipulation of Plasmodium spp. will facilitate a more rational approach to vaccine and drug design.
History
Journal
Annual review of microbiologyVolume
54Pagination
157 - 185Publisher
Annual ReviewsLocation
Palo Alto, Calif.Publisher DOI
ISSN
0066-4227Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2000, Annual ReviewsUsage metrics
Categories
Keywords
AnimalsDrug ResistanceHumansMalariaMutagenesisPlasmodiumTransfectionScience & TechnologyLife Sciences & BiomedicineMicrobiologygenetic systemgene targetingtransgene expressionGREEN FLUORESCENT PROTEINPLASMODIUM-FALCIPARUM MALARIAHUMAN DIHYDROFOLATE-REDUCTASERED-BLOOD-CELLSTOXOPLASMA-GONDIITRYPANOSOMA-BRUCEIPYRIMETHAMINE RESISTANCEHOMOLOGOUS RECOMBINATIONSTABLE TRANSFECTIONDIHYDROPTEROATE SYNTHETASEMicrobiology
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