sinclair-omega3fattyacid-2010.pdf (677.36 kB)
The omega-3 fatty acid, DHA, decreases neuronal cell death in association with altered zinc transport
journal contribution
posted on 2010-02-01, 00:00 authored by Cenk SuphiogluCenk Suphioglu, Damitha De Mel, L Kumar, Nadia Sadli, David Freestone, Agnes MichalczykAgnes Michalczyk, Andrew SinclairAndrew Sinclair, Leigh AcklandLeigh AcklandDocosahexaenoic acid (DHA) is the major polyunsaturated fatty acid in neuronal cell membranes. We hypothesize that DHA induces a decrease in neuronal cell death through reduced ZnT3 expression and zinc uptake. Exposure of M17 cells to DHA-deficient medium increased the levels of active caspase-3, relative to levels in DHA-replete cells, confirming the adverse effects of DHA deficiency in promoting neuronal cell death. In DHA-treated M17 cells, zinc uptake was 65% less and ZnT3 mRNA and protein levels were reduced in comparison with DHA-depleted cells. We propose that the neuroprotective function of DHA is exerted through a reduction in cellular zinc levels that in turn inhibits apoptosis.
History
Journal
FEBS lettersVolume
584Issue
3Pagination
612 - 618Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0014-5793eISSN
1873-3468Language
engPublication classification
C1 Refereed article in a scholarly journal; C Journal articleCopyright notice
2009, ElsevierUsage metrics
Keywords
DHAomega-3 fatty acidzincZnT3M17 human neuronal cellapoptosisScience & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyBiophysicsCell BiologyFATTY-ACID COMPOSITIONDOCOSAHEXAENOIC ACIDALZHEIMERS-DISEASENEURODEGENERATIVE DISORDERSSYNAPTIC VESICLESTRANSGENIC MICEAMYLOID-BETAMOUSE MODELBRAINEvolutionary Biology
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