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Theoretical modeling and experimental validation of surface stress in thrombin aptasensor
journal contribution
posted on 2014-12-01, 00:00 authored by Yang Lim, Abbas KouzaniAbbas Kouzani, Akif KaynakAkif Kaynak, Xiujuan Dai, Guy Littlefair, Wei DuanWei DuanAdsorption of target molecules on the immobilized microcantilever surface produced beam displacement due to the differential surface stress generated between the immobilized and non-immobilized surface. Surface stress is caused by the intermolecular forces between the molecules. Van der Waals, electrostatic forces, hydrogen bonding, hydrophobic effect and steric hindrance are some of the intermolecular forces involved. A theoretical framework describing the adsorption-induced microcantilever displacement is derived in this paper. Experimental displacement of thrombin aptamer-thrombin interactions was carried out. The relation between the electrostatic interactions involved between adsorbates (thrombin) as well as adsorbates and substrates (thrombin aptamer) and the microcantilever beam displacement utilizing the proposed mathematical model was quantified and compared to the experimental value. This exercise is important to aid the designers in microcantilever sensing performance optimization.
History
Journal
IEEE Transactions on NanobioscienceVolume
13Issue
4Pagination
384 - 391Publisher DOI
ISSN
1536-1241Publication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2014, Institute of Electrical and Electronics Engineers Inc.Usage metrics
Keywords
Science & TechnologyLife Sciences & BiomedicineBiochemical Research MethodsNanoscience & NanotechnologyBiochemistry & Molecular BiologyScience & Technology - Other TopicsAtomisticcontinuumintermolecular forcesmathematical modelmicrocantileversurface stressSELF-ASSEMBLED MONOLAYERSBINDINGRECONSTRUCTIONRECOGNITIONRECEPTORADATOMSORIGINGROWTHSU-8MICROCANTILEVER SENSORSDNAPROTEINSNANOMECHANICSHYBRIDIZATIONMOLECULESArtificial Intelligence and Image Processing
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