craig-transcriptionwithin-2003.pdf (378.57 kB)
Transcription within a functional human centromere
journal contribution
posted on 2003-08-01, 00:00 authored by Richard Saffery, Huseyin Sumer, Sara Hassan, Lee H Wong, Jeffrey CraigJeffrey Craig, Kazuo Todokoro, Melissa Anderson, Angela Stafford, K H Andy ChooRecent data in yeast and Drosophila suggest a domain-like centromere structure with a modified chromatin core and flanking regions of heterochromatin. We have analyzed a functional human centromere and defined a region of increased chromosome scaffold/matrix attachment that overlaps three other distinct and nonoverlapping domains for constitutive centromere proteins CENP-A and CENP-H, and heterochromatin protein HP1. Transcriptional competency is intact throughout the S/MAR-enriched region and within the CENP-A- and CENP-H-associated chromatin. These results provide insights into the relationship between centromeric chromatin and transcriptional competency in vivo, highlighting the permissibility of transcription within the constitutively modified, nonheterochromatic chromatin of a functional eukaryotic centromere.
History
Journal
Molecular cellVolume
12Issue
2Pagination
509 - 516Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
Link to full text
ISSN
1097-2765Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2003, Cell PressUsage metrics
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AnimalsAutoantigensCentromereCentromere Protein AChromatinChromosomal Proteins, Non-HistoneChromosomes, Artificial, BacterialChromosomes, Human, Pair 10DNA-Binding ProteinsDrosophilaGene Expression RegulationHepatocyte Nuclear Factor 1Hepatocyte Nuclear Factor 1-alphaHepatocyte Nuclear Factor 1-betaHeterochromatinHumansHybrid CellsModels, GeneticNuclear ProteinsProtein BindingProtein Structure, TertiaryTranscription FactorsTranscription, GeneticScience & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyCell BiologyDETECTABLE ALPHA-SATELLITEMETAPHASE CHROMOSOMESCENP-HPROTEINNEOCENTROMEREDNAORGANIZATIONDOMAIN
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