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Unexpected improvement in stability and utility of cytochrome c by solution in biocompatible ionic liquids
journal contribution
posted on 2006-08-20, 00:00 authored by K Fujita, Maria ForsythMaria Forsyth, D R MacFarlane, R W Reid, G D ElliottProteins generally are only stable in vitro for short periods of time. This results in challenges during isolation and purification of recombinant proteins and reduces the shelf life of protein-based pharmaceuticals. Here we show that certain novel, biocompatible ionic liquids provide a stabilizing solvent for proteins, for example, cytochrome c, such that structure and activity are maintained even after 6 months of storage at room temperature. Normally, this protein would be rendered inactive after only 1 week in buffered aqueous solution. The effect of the ionic liquid solvent appears to be related to protection against hydrolysis.
History
Journal
Biotechnology and bioengineeringVolume
94Issue
6Pagination
1209 - 1213Publisher
WileyLocation
Hoboken, N.J.Publisher DOI
ISSN
0006-3592eISSN
1097-0290Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2006, WileyUsage metrics
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