File(s) under permanent embargo
Van de Graaff generator for capillary electrophoresis
journal contribution
posted on 2017-09-29, 00:00 authored by Seung Jae Lee, Eric R Castro, Rosanne GuijtRosanne Guijt, Mark D Tarn, Andreas ManzA new approach for high voltage capillary electrophoresis (CE) is proposed, which replaces the standard high voltage power supply with a Van de Graaff generator, a low current power source. Because the Van de Graaff generator is a current-limited source (10μA), potentials exceeding 100kV can be generated for CE when the electrical resistance of the capillary is maximized. This was achieved by decreasing the capillary diameter and reducing the buffer ionic strength. Using 2mM borate buffer and a 5μm i.d. capillary, fluorescently labeled amino acids were separated with efficiencies up to 3.5 million plates; a 5.7 fold improvement in separation efficiency compared to a normal power supply (NPS) typically used in CE. This separation efficiency was realized using a simple set-up without significant Joule heating, making the Van de Graaff generator a promising alternative for applying the high potentials required for enhancing resolution in the separation and analysis of highly complex samples, for example mixtures of glycans.
History
Journal
Journal of chromatography AVolume
1517Pagination
195 - 202Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
eISSN
1873-3778Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2017, ElsevierUsage metrics
Categories
No categories selectedKeywords
High Voltage capillary electrophoresisHigh separation efficiencyHigh separation resolutionVan de Graaff generatorAmino AcidsBuffersElectrophoresis, CapillaryPolysaccharidesScience & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsChemistry, AnalyticalBiochemistry & Molecular BiologyChemistryBACKGROUND ELECTROLYTE SOLVENTELECTRIC-FIELD STRENGTHSINITIAL VOLTAGE RAMPZONE-ELECTROPHORESISSEPARATION EFFICIENCYPEAK DISPERSIONPLATE HEIGHTTEMPERATUREETHANOLACID
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC