Deakin University
Browse

File(s) not publicly available

Vectors for Cu2+-inducible production of glutathione S-transferase-fusion proteins for single-step purification from yeast

journal contribution
posted on 1994-04-01, 00:00 authored by Alister WardAlister Ward, L A Castelli, I G Macreadie, A A Azad
We describe six new yeast episomal vectors which encode glutathione S-transferase (GST) affinity tags. These allow for the production of GST-fusion proteins in Saccharomyces cerevisiae under the control of the CUP1 promoter. Affinity chromatography with glutathione-Sepharose permits convenient purification of the fusion protein from a yeast lysate. The presence of a protease cleavage site facilitates subsequent removal of the GST tag. The expression and single-step purification of both GST and a functional GST-metallothionein fusion from yeast are shown as an example of the application of these vectors.

History

Journal

Yeast

Volume

10

Issue

4

Pagination

441 - 449

Publisher

John Wiley & Sons

Location

Chichester, Eng.

ISSN

0749-503X

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

1994, John Wiley & Sons