ward-zebrafishgranulocyte-2019.pdf (3.93 MB)
Zebrafish granulocyte colony-stimulating factor receptor maintains neutrophil number and function throughout the life span
journal contribution
posted on 2019-02-01, 00:00 authored by Faiza BasheerFaiza Basheer, Parisa Rasighaemi, Clifford LiongueClifford Liongue, Alister WardAlister WardGranulocyte colony-stimulating factor receptor (G-CSFR), encoded by the CSF3R gene, represents a major regulator of neutrophil production and function in mammals, with inactivating extracellular mutations identified in a cohort of neutropenia patients unresponsive to G-CSF treatment. This study sought to elucidate the role of the zebrafish G-CSFR by generating mutants harboring these inactivating extracellular mutations using genome editing. Zebrafish csf3r mutants possessed significantly decreased numbers of neutrophils from embryonic to adult stages, which were also functionally compromised, did not respond to G-CSF, and displayed enhanced susceptibility to bacterial infection. The study has identified an important role for the zebrafish G-CSFR in maintaining the number and functionality of neutrophils throughout the life span and created a bona fide zebrafish model of nonresponsive neutropenia.
History
Journal
Infection and immunityVolume
87Issue
2Article number
e00793-18Pagination
1 - 11Publisher
American Society for MicrobiologyLocation
Washington, D.C.Publisher DOI
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eISSN
1098-5522Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2019, American Society for MicrobiologyUsage metrics
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