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Antiarthritic and chondroprotective activity of Lakshadi Guggul in novel alginate-enclosed chitosan calcium phosphate nanocarriers
journal contribution
posted on 2023-10-26, 04:21 authored by Rasika SamarasingheRasika Samarasinghe, Rupinder Kanwar, K Kumar, Jagat KanwarAim:
This study aimed to evaluate the antiarthritic and chondroprotective potentials of Lakshadi Guggul (LG) and Cissus quadrangularis encapsulated in novel alginate-enclosed chitosan-calcium phosphate nanocarriers (NCs) both in vitro in primary human chondrocytes and in vivo in mice with collagen-induced arthritis.
Materials & methods:
Chondrocytes exposed to IL-1beta and osteoarthritis chondrocytes grown in an ex vivo inflammation-based coculture were incubated with different concentrations of herbals, and cell modulatory activities were determined. For in vivo studies, herbals and their encapsulated nanoformulations were administered orally to DBA/1 mice with collagen-induce arthritis.
Results:
C. quadrangularis and LG showed enhanced chondroprotective and proliferative activity in IL-1beta-exposed primary chondrocytes, with LG showing the highest therapeutic potency. LG increased viability, proliferative and mitogenic activity, and inhibited cell apoptosis and mitochondrial depolarization. In vivo studies with LG and alginate-enclosed chitosan-calcium phosphate LG NCs revealed cartilage regenerative activity in those administered with the nanoformulation. The NCs were nontoxic to mice, reduced joint swelling and paw volume, and inhibited gene expression of MMPs and cytokines.
Conclusion:
The promising results from this study reveal, for the first time, the novel polymeric NC encapsulating LG as a potential therapeutic for rheumatic diseases. Original submitted 10 October 2013; Revised submitted 13 December 2013.
Materials & methods:
Chondrocytes exposed to IL-1beta and osteoarthritis chondrocytes grown in an ex vivo inflammation-based coculture were incubated with different concentrations of herbals, and cell modulatory activities were determined. For in vivo studies, herbals and their encapsulated nanoformulations were administered orally to DBA/1 mice with collagen-induce arthritis.
Results:
C. quadrangularis and LG showed enhanced chondroprotective and proliferative activity in IL-1beta-exposed primary chondrocytes, with LG showing the highest therapeutic potency. LG increased viability, proliferative and mitogenic activity, and inhibited cell apoptosis and mitochondrial depolarization. In vivo studies with LG and alginate-enclosed chitosan-calcium phosphate LG NCs revealed cartilage regenerative activity in those administered with the nanoformulation. The NCs were nontoxic to mice, reduced joint swelling and paw volume, and inhibited gene expression of MMPs and cytokines.
Conclusion:
The promising results from this study reveal, for the first time, the novel polymeric NC encapsulating LG as a potential therapeutic for rheumatic diseases. Original submitted 10 October 2013; Revised submitted 13 December 2013.
History
Journal
NanomedicineVolume
9Pagination
819 - 837Location
London, EnglandPublisher DOI
ISSN
1743-5889Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2014, Future MedicineUsage metrics
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No categories selectedKeywords
Lakshadi Guggulantiarthritic and chondroprotective potentialarthritisrheumatic diseasesScience & TechnologyLife Sciences & BiomedicineBiotechnology & Applied MicrobiologyNanoscience & NanotechnologyScience & Technology - Other Topicsalginateanti-inflammatoryantiarthriticchitosanCissus quadrangulariscollagen-induced arthritisnanocarrieroral deliveryosteoarthritisADJUVANT-INDUCED ARTHRITISTERMINALIA-ARJUNA BARKNF-KAPPA-BWITHANIA-SOMNIFERARHEUMATOID-ARTHRITISCARTILAGE DESTRUCTIONTRANSCRIPTION FACTORSARTICULAR-CARTILAGEGENE-EXPRESSION
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