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cDNA sequences of GHF9 endo-β-1,4-glucanases in terrestrial Crustacea
journal contribution
posted on 2018-02-05, 00:00 authored by Michael Gray, Stuart Linton, Ben AllardyceBen AllardyceThis study aimed to sequence and identify a glycosyl hydrolase family 9 (GHF9) endo-β-1,4-glucanase expressed in the midgut gland of the herbivorous gecarcinid land crab, Gecarcoidea natalis. Hence this would explain the gene responsible for the production of previously purified and characterised endo-β-1,4-glucanases. Three different transcripts, two complete and one partial were sequenced from cDNA and an open reading frame of 1383bp was produced. Translated, this would produce a putative protein of 460 amino acid residues, including a 16 amino acid residue signal peptide. The mature protein (without signal peptide) is predicted to have a molecular mass of 47.6-47.7kDa; this closely matches the molecular mass (47.4kDa) of one of the three endo-β-1,4-glucanase/lichenase enzymes purified previously from G. natalis. It is therefore proposed that the gene described here encodes one of the previously characterised enzymes. The presence of multiple transcripts suggests gene duplication. To confirm that the gene is widely expressed within the Crustacea, cDNA encoding a GHF9 endo-β-1,4-glucanase was also sequenced in diverse crustaceans, the deposit feeding soldier crab, Mictyris platycheles and the terrestrial hermit crabs, Coenobita purlatus and C. brevimanus. An open reading frame of 1356bp was sequence from M. platycheles, while an incomplete open reading frames of 1384 and 1523bp were respectively sequenced from Coenobita brevimanus and C. perlatus. The midgut gland of M. platycheles contained activity (0.704±0.218μmol reducing sugars produced. min-1·mg-1 tissue wet weight) of a 26.3±0.3(5) endo-β-1,4-glucanase isozyme (determined from activity staining). These species, particularly M. platycheles does not consume and digest significant amounts of plant cellulose. This implies that the ancestral enzyme is not a cellulase, but rather it may be involved in hydrolysing cellulose like polysaccharides within other organisms such as algae.
History
Journal
GeneVolume
642Pagination
408 - 422Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0378-1119eISSN
1879-0038Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2017, Elsevier B.V.Usage metrics
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Keywords
CellulaseDigestive biochemistryEndo-β-1,4-glucanaseGecarcoidea natalisLand crabLichenaseScience & TechnologyLife Sciences & BiomedicineGenetics & HeredityEndo-beta-1,4-glucanaseGECARCINID LAND CRABENDOGENOUS PRODUCTIONGECARCOIDEA-NATALISCELLULASE ACTIVITYREDCLAW CRAYFISHENZYMESFOODPURIFICATIONASSIMILATIONORIGINPhysiologyGenetics
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