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miRNA modulation of SOCS1 using an influenza A virus delivery system

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journal contribution
posted on 2014-09-01, 00:00 authored by Leonard Izzard, Siying Ye, K Jenkins, Yingju Xia, M Tizard, John StambasJohn Stambas
Difficulties associated with efficient delivery and targeting of miRNAs to cells is hampering the real world application of miRNA technology. This study utilized an influenza A-based delivery system to express miR-155 in order to knockdown SOCS1 mRNA. Using qPCR and dual luciferase technology we show that miR-155 delivery resulted in a significant increase in cellular miR-155 which facilitated a downregulation of SOCS1 gene expression and a functional increase in IL-6 and IFN-β cytokines.

History

Journal

Journal of general virology

Volume

95

Issue

Pt 9

Pagination

1880 - 1885

Publisher

Society for General Microbiology

Location

Reading, England

ISSN

0022-1317

eISSN

1465-2099

Language

eng

Publication classification

C Journal article; C1 Refereed article in a scholarly journal

Copyright notice

2014, Society for General Microbiology